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首页> 外文OA文献 >The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA.
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The external transcribed spacer and preceding region of Xenopus borealis rDNA: comparison with the corresponding region of Xenopus laevis rDNA.

机译：非洲爪蟾rDNA的外部转录间隔区和先前区域：与非洲爪蟾rDNA的相应区域比较。

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摘要

We report sequence data from a cloned rDNA unit from Xenopus borealis, extending leftwards from the 18S gene to overlap a region previously sequenced by R. Bach, B. Allet and M. Crippa (Nucleic Acids Research 9, 5311-5330). Comparison with data from other species of Xenopus leads to the inference that the transcription initiation site in X.borealis is in the newly sequenced region and not, as was previously thought, in the region sequenced earlier. The X.borealis external transcribed spacer thus defined is some 612 nucleotides long, about 100 nucleotides shorter than in X.laevis. The X.borealis and X.laevis external transcribed spacers show a pattern of extensive but interrupted sequence divergence, with a large conserved tract starting about 100 nucleotides downstream from the transcription initiation site and shorter conserved tracts elsewhere. The regions in between the conserved tracts differ in length between the respective external transcribed spacers indicating that insertions and deletions have contributed to their divergence, as previously inferred for the internal transcribed spacers. Much of the overall length difference is in the region flanking the 18S gene, where there are also length microheterogeneities in X.laevis rDNA. As in X.laevis, the transcribed spacer sequences flanking the 18S gene in X.borealis contain no major tracts of mutual complementarity. The accumulated data on transcribed spacers in Xenopus render it unlikely that processing of ribosomal precursor RNA involves interaction between the regions flanking 18S RNA.
机译：我们报告了来自非洲爪蟾的克隆rDNA单元的序列数据，该序列从18S基因向左延伸，以覆盖先前由R. Bach，B。Allet和M. Crippa（Nucleic Acids Research 9，5311-5330）测序的区域。与非洲爪蟾其他物种的数据进行比较，可以得出结论，北欧双歧杆菌的转录起始位点在新测序的区域，而不是先前认为的在先前测序的区域。如此定义的北极X.外部转录间隔区长约612个核苷酸，比X.laevis中短约100个核苷酸。 X.borealis和X.laevis外部转录的间隔区显示出广泛但被中断的序列差异的模式，其中一个大的保守区在转录起始位点下游约100个核苷酸处开始，而其他地方的保守区较短。在保守区域之间的区域在各个外部转录间隔区之间的长度不同，这表明插入和缺失已经促进了它们的发散，如先前对于内部转录间隔区所推断的。总的长度差异大部分在18S基因的侧翼区域，在X.laevis rDNA中也存在长度微异质性。如在X.laevis中一样，在X.borealis中18S基因侧翼的转录间隔区序列不包含相互互补的主要片段。在非洲爪蟾中转录间隔区的积累数据使得核糖体前体RNA的加工不太可能涉及18S RNA两侧区域之间的相互作用。

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